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Table 2 Gradient conditions of screening method 03 on the Waters 2695

From: Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC

Time

Flow

%Phase C1

%Phase A

Curve

0.0

0.20

10

90

6

1.0

0.20

10

90

6

40.0

0.20

100

0

6

45.0

0.30

100

0

6

50.0

0.20

10

90

3

58.0

0.20

10

90

6

59.0

0.20

10

90

6

78.0

0.20

100

0

6

  1. 1Phase C = methanol, Phase A = water with 0.1% formic acid.