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Table 1 Gradient conditions of screening method 02 on the Waters 2695

From: Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC

Time

Flow

%Phase A1

%Phase B

Curve

0.0

0.20

90

10

6

1.0

0.20

90

10

6

40.0

0.20

0

100

6

45.0

0.20

0

100

6

50.0

0.20

90

10

3

58.0

0.20

90

10

6

59.0

0.20

90

10

6

  1. 1Phase A = water with 0.1% formic acid, Phase B = acetonitrile.