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Table 1 Gradient conditions of screening method 02 on the Waters 2695

From: Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC

Time Flow %Phase A1 %Phase B Curve
0.0 0.20 90 10 6
1.0 0.20 90 10 6
40.0 0.20 0 100 6
45.0 0.20 0 100 6
50.0 0.20 90 10 3
58.0 0.20 90 10 6
59.0 0.20 90 10 6
  1. 1Phase A = water with 0.1% formic acid, Phase B = acetonitrile.