Figure 5
TLC plates eluted in CEF solvent system (chloroform:ethyl acetate:formic acid = 5:4:1) and sprayed with Enterococcus faecalis (left) and vanillin-sulphuric acid spray reagent (right). The solvent fronts and origins are marked with pencil lines at the top and bottom of the TLC plates respectively. *Extracts: 1 = DEE extract, 2 = DCM/MeOH extract, 3 = EtOAc extract, 4 = EtOH extract, 5 = H2O extract.