Figure 3
From: Molecular mechanism of apoptosis induction in skin cancer cells by the centipedegrass extract
Effect of CGE on the PI3K/AKT/ GSK-3β pathway. (A) CGE inhibited the constitutively active PI3K/AKT/glycogen synthase kinase-3β (GSK-3β) signaling pathway in skin cancer cell lines in a dose-dependent manner. B16F1 and SKMEL-5 cells were treated with CGE at the indicated doses for 48 h. Equal amounts of protein from each sample were separated by SDS-PAGE and immunoblotted with p-AKT (Ser 473), AKT, and GAPDH. (B) B16F1 and SKMEL-5 cells were treated with 50 μg·mL-1 CGE for 8, 24, and 48 h. Proteins from each sample were separated by SDS-PAGE and immunoblotted with p-PI3K, p-AKT (Thr 308), p-AKT (Ser 473), p-GSK-3β (Ser 9), GSK-3β, p-BAD (Ser 136), BAD, and GAPDH.