Figure 1
From: Molecular mechanism of apoptosis induction in skin cancer cells by the centipedegrass extract
Centipedegrass extract (CGE) induced cytotoxicity through G 2 /M cell cycle arrest and both early and late apoptosis. (A) Cell viability of B16F1 and SKMEL-5 skin cancer cells after CGE treatment by MTT assay. (B) Double staining with annexin V-FITC and propidium iodide (PI) demonstrated an increase in the percentage of apoptotic cells (early and late) when skin cancer cells (SKMEL-5) were treated with the indicated doses of CGE for 48 h. (C) Flow cytometry analysis of cell cycle distribution in CGE-treated B16F1 and SKMEL-5 skin cancer cells. (D) Data are presented as mean ± SD for at least three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the control.