Figure 4
From: Induction of apoptosis in cancer cell lines by the Red Sea brine pool bacterial extracts
Western blot analysis of PARP-1 cleavage, phosphorylated γH2AX, Caspase-8 and −9 in HeLa cells after treatment with marine bacterial extracts. Protein lysates of HeLa cells treated with 500 μg/mL of each extract for 12 and 24 h were subjected to western blotting probing for cleaved PARP-1 (cl-PARP-1), procaspase-9, cleaved caspase-8 and γH2Ax. Cells treated with 400 nM docetaxel for 24 h were used as a positive (+ve) control, Untx represent untreated control and β-tubulin was used as a loading control.