MiR-181a was induced by bufalin in PC-3 cells. A. Expression of 10 oncogenic miRNAs in PC-3 cellstreated withbufalin at a concentration of 15 μM for 24 hours. B. Expression of 10 tumor-suppressor miRNAs in PC-3 cells treated with bufalin at 15 μM for 24 hours. C. Expression of miR-181a in PC-3 cells treated with bufalinat 0, 5, 10, and 15 μM for 24 hours. Vertical axes indicate fold-changes in expression of miRNAs in cells, with and without bufalin treatment. All data were from three replications; error bar indicatedstandard error of each measurement.