The effect of Dangui-Jakyak-San (DJS) on ischemia-induced changes of GSK3β signaling. Using Western blot assay, changes of GSK3β signaling were examined. (A) Representative photographs of Western blotting. Ratio of pAkt to Akt (B), ratio of pGSK3β to GSK3β (C), and ratio of β-catenin to tubulin (D) immunoreactivity in the hippocampal tissue were analyzed. Data are presented as mean ± S.E.M (n = 4). *P < 0.05, compared with sham group (one-way ANOVA followed by Student Newman-Keuls’s post-hoc test).