Figure 3
The effect of Dangui-Jakyak-San (DJS) on ischemia-induced neurogenesis. To test newborn cell survival and neurogenesis, Ki67 or DCX immunostaining was conducted. (A) Representative of microphotographs of Ki67- and DCX-, and nissl staining-positive cells in the hippocampal DG region (Bar = 10, 25 or 50 μm). Arrows indicate immunopositive cells. (B) The number of Ki67-immunopositive cells, (C) the number of DCX-immunopositive cells in the DG, and (D) severity of neuronal damage in the CA1 region were analyzed. (E-G) To test effect of DJS on ischemia-induced neural differentiation, double-immunostainings were conducted. (E) Representative of microphotographs of BrdU/NeuN-, BrdU/DCX-, and BrdU/GFAP-immunopositive cells in the hippocampal DG region. Bar = 30 μm. (F) BrDU-immunopositive cells, BrDU/NeuN-immunopositive cells, BrDU/DCX-immunopositive cells or BrDU/GFAP-immunopositive cells were counted. (G) Proportion of BrdU/NeuN-, BrdU/DCX-, and BrdU/GFAP-immunopositive cells to BrdU-immunopositive cells in the hippocampal DG region was analyzed. Data are presented as mean ± S.E.M (n = 6/group). *P < 0.05, compared with sham group. #P < 0.05, compared with BCCAO group (one-way ANOVA followed by Student Newman-Keuls’s post-hoc test).