Effect of TD on control and treated Huh7 cells. (a) Morphological changes of Huh7 cells in the presence or absence of TD. Huh7 cells were treated with TD at the concentrations of 50 and 100 μg/ml for 48 h (magnification x40). (b) Nuclear changes after treatment with TD (50 and 100 μg) for 48 h, the Huh7 cells were stained with DAPI. (c) Huh7 cells were treated with 50 and 100 μg/ml of TD for 48 h, stained by Rh 123.