Ardipusilloside I induces apoptosis of the Mc3 cell line. (A) Mc3 cells were treated with NS. (B) Mc3 cells were treated with 10.0 μg/ml ardipusilloside I for 24 h. (C) Mc3 cells were treated with 10.0 μg/ml ardipusilloside I for 48 h. Annexin binding and propidium iodide (PI) staining were analyzed by FACScan. Q3: viable cells; Q4: early apoptotic cells; Q2: late apoptotic cells. (D) 10.0 μg/ml ardipusilloside I treatment greatly enhanced the apoptosis of Mc3 cells (*P < 0.0001, **P < 0.0001).