Morphological observation with DAPI staining by fluorescence microscope for MCF7 cells at 24 hours (magnification 400×). Arrows indicate signs of nuclear shrinkage and chromatin condensation. DNA samples in the untreated cells were homogenously stained and less intense compared to those in treated cells. Note that the cells were treated with the following extracts/sub-fractions: a = control, b = leaf hexane, c = leaf chloroform, d = fraction LC4, e = Sub-fraction VLC9 and f = positive control. Figures shown were obtained from at least three independent experiments with similar parameter.