Cdk5 activity and level after spinal ischemia-reperfusion injury. Cdk5 activity was significantly elevated after spinal ischemia-reperfusion injury. The administration of BYHWD and roscovitine greatly inhibited Cdk5 activity (A). The similar results were confirmed in Western blot, showing that spinal ischemia-reperfusion resulted in an elevation of Cdk5 level, and that BYHWD and roscovitine inhibited Cdk5 level (B). β-Actin was used as a loading control. *#: P < 0.05, vs. SI + Saline group. ** P < 0.01, vs. Control group SI + Saline: Spinal ischemia + saline. SI + BYHWD: Spinal ischemia + BYHWD. SI + R: Spinal ischemia + Roscovitine.