Figure 3

Cucurbitacin I dose response curves. a. Effects of cucurbitacin I on PDGF-BB stimulation of human leptomeningeal cell DNA synthesis. Fetal leptomeningeal cells (L2) were treated with PDGF-BB without or with cucurbitacin I (50, 100 or 200 nM) for 72 hrs. CyQUANT analysis of DNA measured by fluorescence at 480/520 nm. Changes in DNA fluorescence correlate with cell proliferation. PDGF = Platelet derived growth factor-BB. * P < 0.05, *** P < 0.005, ***** P < 0.001. b. Effects of cucurbitacin on cerebrospinal fluid stimulation of meningioma cell DNA synthesis. WHO grade I (M2) and grade II (M6) meningioma cells were treated with CSF without or with cucurbitacin (25 nM, 75 nM or 250 nM) for 72 hrs. CyQUANT analysis of DNA measured by fluorescence at 480/520 nm. Changes in DNA fluorescence correlate with cell proliferation. CSF = cerebrospinal fluid * P < 0.05, *** P < 0.005, **** P < 0.001, ***** P < 0.0005.