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Table 3 DNA migration in the comet assay observed in Wistar rat colon treated with different doses of the C. langsdorffii extract and/or DMH and their respective controls

From: Chemopreventive effect of Copaifera langsdorffii leaves hydroalcoholic extract on 1,2-dimethylhydrazine-induced DNA damage and preneoplastic lesions in rat colon

Treatments (mg/kg)

Comet class*

*Score

Reduction

 

0

1

2

3

 

(%)

Control

66.2 ± 4.3

23.2 ± 5.7

7.3 ± 2.3

3.3 ± 1.8

47.8 ± 6.4

-

DMSO

65.3 ± 3.9

21.2 ± 4.4

8.3 ± 2.4

3.7 ± 2.2

48.8 ± 4.8

-

80

79.7 ± 5.1

14.7 ± 6.3

5.0 ± 2.6

0.7 ± 0.8

26.7 ± 6.0

-

DMH

13.2 ± 2.1

26.2 ± 5.3

33.5 ± 5.2

27.3 ± 3.7

175.2 ± 9.6a

-

DMSO + DMH

11.5 ± 1.6

23.8 ± 9.5

32.8 ± 8.3

28.7 ± 4.3

175.5 ± 11.7a

-

20 + DMH

10.8 ± 4.5

40.2 ± 3.6

25.0 ± 6.4

22.3 ± 4.3

157.2 ± 12.2a,b

14.3

40 + DMH

19.3 ± 6.3

34.0 ± 4.3

29.7 ± 3.6

16.8 ± 5.9

143.8 ± 15.1a,b

24.7

80 + DMH

23.3 ± 6.9

40.8 ± 7.1

20.8 ± 3.5

15.0 ± 5.9

125.8 ± 12.7a,b,c

38.8

  1. A total of 600 cells were examined per treatment; DMH, 1,2-dimethylhydrazine (40 mg/kg). The numbers 20, 40, and 80 correspond to the extract dose in mg/kg.
  2. *Values are the mean ± standard deviation.
  3. aSignificantly different from the control (P < 0.05).
  4. bSignificantly different from the DMH group (P <0.05).
  5. cSignificantly different from the 20 + DMH group (P < 0.05).