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Table 1 Minimum inhibitory concentration (MIC) of the extract, fractions and compounds (1–8) from E. robustum against fungi (C.a, C.A, A.f, C.n) and bacteria (S.a, P.a, E.f, E.c)

From: Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae)

Samples Fungi (MIC values) Bacteria (MIC values)
C.a C.A A.f C.na Average S.a P.a E.f E.c Average
16 h 24 h 16 h 24 h 16 h 24 h 24 h 36 h       
Extract and Fractions (mg/ml)               
Crude extract 1.25 1.25 1.25 1.25 0.16 0.63 0.63 0.63 0.88 0.08 0.31 0.08 0.08 0.14
Fraction A 0.63 0.63 0.16 0.32 0.32 0.63 1.25 1.25 0.65 0.02 0.31 0.04 0.04 0.10
Fraction B 1.25 1.25 0.32 0.63 0.08 0.63 0.08 0.63 0.61 0.16 0.31 0.08 0.08 0.16
Compounds (μg/ml)               
Robusflavone A (1) 250 250 65 65 65 125 250 250 165 250 63 250 250 203.3
Robusflavone B (2) 125 125 65 65 65 125 65 65 88 125 63 63 125 94
Orostachyscerebroside A (3) 250 250 130 250 130 250 250 250 220 125 63 125 125 109.5
Stigmasterol (4) 125 125 125 125 65 65 125 125 110 125 63 250 250 172
1-O-Heptatriacontanoyl glycerol (5) 250 250 65 65 125 125 250 250 173 125 63 125 125 109.5
Eicosanoic acid (6) 250 250 125 125 125 125 > 250 > 250 188 63 63 125 125 94
3-O-β-D-Glucopyranoside of sitosterol (7) 125 125 125 125 65 125 65 125 110 125 63 63 125 94
6-Prenylpinocembrin (8) 125 125 65 65 125 250 > 250 > 250 157 31.3 63 63 63 55.1
Controls (μg/ml)               
Amp B 30 30 10 30 65 125 > 250 > 250 99 - - - - -
Gen - - - - - - - - - < 3.91 < 3.91 < 3.91 < 3.91 < 3.91
DMSOb na na na na na na na na   na na na na  
  1. C.a: Candida albicans (isolate), C.A: Candida albicans (ATCC), C.n: Cryptococcus neoformans, A.f: Aspergillus fumigatus, E.c: Escherichia coli, E.f: Enterococcus faecalis, S.a: Staphylococcus aureus, P.a: Pseudomonas aeruginosa, Amp B: Amphotericin B, Gen: Gentamicin, aWith this microorganism little reaction was observed after 16 h and values were measured after 24 and 36 h, bThe final concentration of DMSO used was less than 10%, na: not active.
  2. The results are the means of three replicates for fungi and two replicates for bacteria and the standard deviation was zero.