| Gradient |  |
---|---|---|
Sub-fractions (SF) | (Percent ratio of eluent: hexane to ethyl acetate) | IC50 |
 |  | (μg/mL) |
1 | 85:15 | NLI |
2 | 83:17 | 42.06 ± 0.67 |
3 | 81:19 | 35.17 ± 4.63 |
4 | 78:22 | 31.61 ± 6.52 |
5 | 75:25 | 40.55 ± 7.24 |
6 | 71:29 | NLI |
7 | 67:33 | NLI |
8 | 63:37 | NLI |
9 | 59:41 | 3.96 ± 0.07 |
10 | 55:45 | 4.98 ± 0.50 |
11 | 50:50 | 11.92 ± 1.29 |
Doxorubicin |  | 4.31 ± 0.06 |