Skip to main content

Table 1 Sequences of primers and PCR conditions for semi-quantitative RT-PCR

From: Polygonum cuspidatum inhibits pancreatic lipase activity and adipogenesis via attenuation of lipid accumulation

Gene Sequence (5′-3′) Annealing temperature (°C) Size (bp) Accession No.
ADRP   55 352 NM_00748
Sense CTT GTG TCC TCC GCT TAT GTC AGT
Anti-sense CTG CTC CTT TGG TCT TAT CCA CCA
Perilipin   55 508 NM_175640
Sense CTT TCT CGA CAC ACC ATG GAA ACC
Anti-sense CCA CGT TAT CCG TAA CAC CCT TCA
PPAR-γ   55 220 NM_011146
Sense CCA GAG CAT GGT GGG TTC GCT G
Anti-sense GAG CTG ACC CAA TGG TTG CTG
C/EBP-α   55 238 NM_007678
Sense AGG TGG TGG AGT TGA CCA GT
Anti-sense CAG CCT AGA GAT CCA GCG AC