Figure 2

Effect of β-sitosterol (ST) on G0/G1 phase cell cycle regulators and mitogenic and survival signaling in breast cancer cells. MDA-MB-231 cells were treated with either DMSO control or various doses of β-Sitosterol (60 and 90 μM) for 48 h. At the end of these treatments, cell lysate was prepared and western blot analysis was performed. Membranes were probed with (A) anti-cyclin D1, CDK-4, p21/Cip1, p27/Kip1, and (B) anti-p-Erk1/2, Erk1/2, p-Akt and Akt antibodies followed by peroxidase-conjugated appropriate secondary antibodies, and visualized by ECL detection system. Membranes were striped and re-probed with anti-β actin for loading control.