Figure 2

Effect of ethyl acetate fraction from aged garlic extract on ROS production determined in the presence and absence of Aβ in PC12 cell. PC12 cells were pretreated for 48 h with various concentrations. After 48 h, the cells were treated with 80 μM Aβ for 2 h. The increase of DCF-DA fluorescence was measured by fluorescence microplate reader. Each value represented the means ± SD of triplicates. Different superscripts indicate significant difference among groups at p < 0.05.