The testing results of biomarkers of rats serum with multiplexing sandwich-ELISA method. A. Serum was filled in 96-well plate collected CCD images of biomarkers from a ProArray Analysis System. Respective capture-antibodies are spotted in arrays within each well. 8 spots are for standard curve, each standard sample test in triplicates (left area). 6 group rat’s serum samples displayed the concentration profiles by quantitative chemiluminescence (right area). Samples test in duplicates, control test in duplicates for two different concentrations. B. The analysis results of 3 biomarkers Acrp30, IL-1β, and IL-10 in serum of T2DM rats after Pseudostellaria heterophylla polysaccharide (PF-40) treatment. C. The analysis results of 3 biomarkers CRP, TNF-α and leptin in serum of T2DM rats after Pseudostellaria heterophylla polysaccharide (PF-40) treatment.