Figure 4

Xanthones exerted a protective effect on DNA oxidation induced by an OH ^● generation system. a) Representative ethidium bromide stained DNA-agarose gel. Lane 1, molecular weight markers; lane 2, DNA (20 μg); lane 3, xanthone III (2.5 μM); lane 4, xanthone V (2.5 μM); lane 5, α-mangostin (2.5 μM); lane 6, DNA (20 μg) + OH^●; lanes 7–9 DNA (20 μg) + OH^● + xanthone III (0.5, 1 and 2.5 μM, respectively); lane 10–12 DNA (20 μg) + OH^● + xanthone V (0.5, 1 and 2.5 μM, respectively); lanes 13–15, DNA (20 μg) + OH^● + α-mangostin (0.5, 1 and 2.5 μM, respectively). b) Quantitative representation of the DNA detection. c) Representative ethidium bromide stained DNA-agarose gel. Lane 1, molecular weight markers; lane 2, DNA (20 μg); lane 3, xanthone III (2.5 μM); lane 4, xanthone V (2.5 μM); lane 5, α-mangostin (2.5 μM); lane 6, EtOH; lanes 7, DNA (20 μg) + OH^●; lane 8, DNA (20 μg) + OH^● + xanthone III (2.5 μM); lane 9, DNA (20 μg) + OH^● + xanthone V (2.5 μM); lanes 10, DNA (20 μg) + OH^● + α-mangostin (2.5 μM) and lane 11, DNA (20 μg) + OH^● + EtOH. Data are presented as mean ± S.E.M of 4 independent experiments. ^aP < 0.01 vs. Control, ^bP < 0.01 vs. DNA + OH^●.