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Table 3 IC 50 values obtained by using different cytotoxicity assays- MTT, NRU and LDH release

From: Neurite outgrowth stimulatory effects of culinary-medicinal mushrooms and their toxicity assessment using differentiating Neuro-2a and embryonic fibroblast BALB/3T3

Mushroom/Plant Species Extracts Neuroblastoma 2a cells 3T3 embryonic fibroblast
IC50(mg/ml) after 24 h IC50(mg/ml) after 24 h
MTT NRU LDH MTT NRU LDH
Culinary/medicinal mushroom Pleurotus giganteus Aqueous 4.07 ± 0.67e 6.95 ± 1.00g - 2.16 ± 0.05c 2.67 ± 0.47d -
Pleurotus pulmonarius   2.85 ± 0.06cd 2.13 ± 0.87cdef - 1.75 ± 0.14bc 2.60 ± 0.55d -
Lignosus rhinocerotis (sclerotium)   3.27 ± 0.77d 2.72 ± 0.65ef - 5.63 ± 0.06e 5.93 ± 0.05f -
Lignosus rhinocerotis   2.43 ± 0.12c 1.75 ± 0.18abc - 5.23 ± 0.17e 5.60 ± 0.1f -
(mycelium)
Hericium erinaceus   2.60 ± 0.52cd 2.85 ± 0.56e - 3.43 ± 0.29d 3.53 ± 0.06e -
Ganoderma lucidum   1.35 ± 0.03a 1.52 ± 0.17ab 2.20 ± 0.52ab 1.19 ± 0.06ab 1.35 ± 0.02ab 1.50 ± 0.08a
Ganoderma neo-japonicum   1.17 ± 0.006a 1.11 ± 0.03a 2.00 ± 0.53ab 1.47 ± 0.37ab 1.78 ± 0.56bc 1.58 ± 0.44a
Grifola frondosa 2.72 ± 0.08cd 4.60 ± 1.10f - 6.67 ± 0.87f 7.60 ± 0.1g -
Pleurotus giganteus Ethanol 2.43 ± 0.10c 2.81 ± 0.15e 5.8 ± 0.10c 1.66 ± 0.56bc 1.96 ± 0.42c 1.73 ± 0.48a
Pleurotus pulmonarius   2.30 ± 0.72bc 2.64 ± 0.16def 2.53 ± 0.96bc 1.27 ± 0.06ab 1.48 ± 0.05abc 1.67 ± 0.12a
Hericium erinaceus   2.47 ± 0.50c 1.61 ± 0.51ab 2.10 ± 0.72ab 1.31 ± 0.03ab 1.84 ± 0.57bc 1.47 ± 0.31a
Cordyceps militaris   1.65 ± 0.015ab 1.81 ± 0.10abcd 2.37 ± 0.21b 1.02 ± 0.08a 1.10 ± 0.02a 1.40 ± 0.29a
Medicinal plant Lycium barbarum Aqueous 4.37 ± 1.00e 6.96 ± 0.73g - 8.40 ± 0.72g 7.86 ± 0.06g -
Gingko biloba Ethanol 1.56 ± 0.08ab 1.66 ± 0.11ab 1.43 ± 0.39a 1.56 ± 0.58abc 1.37 ± 0.07ab 1.78 ± 0.07a
Curcumin Compound 1.13 ± 0.01a 1.15 ± 0.01a 1.30 ± 0.16a 1.16 ± 0.06ab 1.23 ± 0.03ab 1.60 ± 0.22a
  1. The data represent the mean ± SD of three determinations. Means not sharing a common letter were significantly different at p < 0.05.