Figure 5
Immunocytochemical staining of neurofilament in N2a cells treated with (a) NGF, (b) G. lucidum , (c) L. rhinocerotis , (d) P. giganteus , (e) G. frondosa , and (f) C. militaris , and untreated cells as control (g). DAPI stains for nuclei blue, while anti-neurofilament 200 kD labeled with FITC stains neuronal cells green. Scale bar represents 20 μm. Arrows indicate neurite outgrowth. Photomicrographs of representative microscope fields were taken with a 20× objective.