Figure 3

Intracellular activity of Ursolic acid (UA), Oleanolic acid (OA) and their combination against M. tuberculosis H37Rv and MDR clinical isolate in the macrophage cell model. Macrophages (cell line J774A.1) were infected (MOI 10:1) with drug-sensitive H37Rv or DR strain and treated with UA, OA, or both together at two concentrations, upper (panel A) and lower (panel B). After several time points, cells were lysed and intracellular bacillus colony forming units (CFU) were determined. In comparison with non-treated control cells, each terpenoid alone had significant antimycobacterial activity at high or low treatment concentrations, but when OA and UA were incubated together, the antimycobacterial activity was more efficient. *P <0.001.