Application of proteomics to identify the target molecules involved in Lonicera japonica-induced photokilling in human lung cancer CH27 cells

Table 1 MS/MS analysis and characteristics of the analyzed spots

Spot Protein	Accession no.	Calculated pI	Theoretical pI/Mw (Da)	Score	Sequence coverage (%)	Changed fold (% control)¹
D1 DJ-1 protein	NP_009193	6.33	6.32 / 19891.05	88	25	40 ± 4
D2 Heat shock protein 27	AAA62175	6.16	5.98 / 22782.52	273	43	46 ± 5
D3 Protein disulfide isomerase	CAA89996	5.85	5.98 / 56782.39	766	40	48 ± 9
D4 Heat shock 70 kDa protein 9	AAH00478	5.59	5.87 / 73680.50	508	28	49 ± 7
D5 Actin, cytoplasmic 1	NP_001092	5.42	5.29 / 41736.73	896	43	41 ± 5
D6 ATP synthase	NP_006347	5.21	5.21 / 18491.21	147	34	48 ± 3
D7 Tropomyosin 3 isoform 2	NP_705935	4.81	4.68 / 32818.79	283	29	45 ± 11
D8 Tropomyosin 3 isoform 4	NP_001036816	4.73	4.73 / 28870.36	283	29	79 ± 5
#1 Protein disulfide isomerase	CAA89996	5.88	5.98 / 56782.39	423	32	383 ± 26
#2 Protein disulfide isomerase	CAA89996	5.70	5.98 / 56782.39	494	32	143 ± 21
#3 Heat shock 70 kDa protein 9B precursor variant	BAD96478	5.60	5.87 / 73680.50	405	25	1594 ± 115
#4 Heat shock 70 kDa protein 8 isoform 1	NP_006588	5.43	5.61 / 53499.58	540	28	366 ± 62
#5 Chaperonin	NP_002147	5.34	5.70 / 61054.64	760	46	145 ± 25
#6 Chaperonin	NP_002147	5.15	5.70 / 61054.64	600	31	386 ± 51
#7 Actin, cytoplasmic 1	NP_001092	5.25	5.29 / 41736.73	336	37	266 ± 10
#8 Actin, cytoplasmic 1	NP_001092	5.15	5.29 / 41736.73	262	39	376 ± 43

¹The expression of protein spots was quantified by AlphaEase image software. All protein changed folds are expressed as the mean percentage of the relative control ± S.D. from three independent experiments.

ISSN: 2662-7671