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Figure 5 | BMC Complementary and Alternative Medicine

Figure 5

From: Application of proteomics to identify the target molecules involved in Lonicera japonica-induced photokilling in human lung cancer CH27 cells

Figure 5

Effects of photoactivated Lonicera japonica on reactive oxygen species (ROS) production in CH27 cells. Cells were loaded with 5-H2DCFDA (5 μM) for 30 min in the dark, washed and then treated with 0.1% DMSO or with 50, 75 or 100 μg/ml Lonicera japonica for 2 h and then irradiated with 0.8 J/cm2 fluence dose. After irradiation, ROS fluorescence was measured using a multiwell plate reader at an excitation wavelength of 485 nm and an emission wavelength of 538 nm. All results are expressed as the mean percentage of control ± S.D. of triplicate determinations from four independent experiments. ** P < 0.01, *** P < 0.001 compared to the corresponding control values.

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