Time course of larvicidal activity A) and loss of spontaneous movements B)of A. simplex by yakuchinone A treatment. Effectof yakuchinone A (10, 100, and 200 μM) for 2, 4, 8, 12, 24, 48 and72 h on third-stage larvae of A. simplex. Vehicle is 0.1% DMSOsolvent. Each value is presented as mean ± SD of threeindividual experiments. Statistically significant,*p < 0.05 indicates a significant difference from theresult for vehicle-treated worms.