Effect of yakuchinone A on H. nana . Treatmentwith various concentrations of yakuchinone A (10, 50, and 100 μM)with incubation times of 2, 4, 6, 12, 24, 48, and 72 h on H.nana, respectively. Time course of effect on oscillation A) andperistalsis B) of H. nana of yakuchinone A presented aspercentages. Vehicle is 0.1% DMSO solvent. Each value is presented asmean ± SD of three individual experiments;*p < 0.05 indicates a significant difference from theresult for vehicle-treated worms.