Expression of Bcl-2 and bax-dependent apoptotic pathway in BCC cells afteryakuchinone A treatment. A) Morphological changes induced by yakuchinoneA in BCC cells. BCC cells were treated with yakuchinone A (20 μM) andvehicle control (DMSO) for 72 h, and then the nuclear was stained withHoechst 33342. Apoptotic cells (arrows) were characterized by cellularshrinkage and rounded cell bodies (phase-contrast-inverted microscopic,200×). Under a fluorescent microscope, apoptotic cells (arrows) werecharacterized by marked nuclear condensation, shrinking and fragmentation(200×). B) Effect of yakuchinone A on bcl-2 and bax expressions.BCC cells were treated with yakuchinone A (20 μM) and vehicle control(DMSO) for 24 and 48 h, and the bcl-2, bax andβ-actin expressions were determined by RT-PCR. C)Expressions of Bcl-2, Bax and PARP on cells after yakuchinone A treatment. BCCcells were treated with (+) or without (-) yakuchinone A (20 μM) for72 h, and the Bcl-2, Bax, PARP and β-actin expression were assessedby Western blotting.