Yakuchinone A inhibits adipocyte differentiation. A) Photomicrograph ofOil Red O stained differentiating 3 T3-L1 cells. Cells were treated withyakuchinone A (5 μM) for eight days. Lipid accumulation was measuredby Oil Red O staining. B) Percentage lipid accumulation was analyzed byquantitative analysis of Oil Red O staining. Data are presented asmean ± SD from three independent experiments;*p < 0.05 indicates significant difference fromvehicle-treated cells. C) The gene expressions of leptin,PPAR γ, and β-actin were determined byRT-PCR.