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Table 2 RP-HPLC-DAD operating conditions for analysis of Samsoeum (SSE)

From: Samsoeum, a traditional herbal medicine, elicits apoptotic and autophagic cell death by inhibiting Akt/mTOR and activating the JNK pathway in cancer cells

Item Condition
Instrument Waters 2695 and 966 photodiode array detector
Software system Waters Empower 1.0 software system
Column RS tech C18 column (5 μm, 4.6 mm × 250 mm)
Column temperature 40°C
Injection volume 20 μL
Mobile phase Water with 0.1% trifluoroacetic acid (TFA)(A) and Acetonitrile (B)
Flow rate 1.0 mL/min
UV wavelength 254 and 280 nm
Time (min) 0.1% TFA (A) Acetonitrile (B)
0 95 5
10 95 5
15 85 15
35 80 20
45 75 25
70 25 75
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