Samsoeum, a traditional herbal medicine, elicits apoptotic and autophagic cell death by inhibiting Akt/mTOR and activating the JNK pathway in cancer cells

Table 2 RP-HPLC-DAD operating conditions for analysis of Samsoeum (SSE)

Item	Condition
Instrument	Waters 2695 and 966 photodiode array detector
Software system	Waters Empower 1.0 software system
Column	RS tech C₁₈ column (5 μm, 4.6 mm × 250 mm)
Column temperature	40°C
Injection volume	20 μL
Mobile phase	Water with 0.1% trifluoroacetic acid (TFA)(A) and Acetonitrile (B)
Flow rate	1.0 mL/min
UV wavelength	254 and 280 nm
Time (min)	0.1% TFA (A)	Acetonitrile (B)
0	95	5
10	95	5
15	85	15
35	80	20
45	75	25
70	25	75

ISSN: 2662-7671