SSE induces dose- and time-dependent cell death in human and murine cancer cell lines. (A) Cells seeded on 96-well plates (5 × 103 cells/well) were treated with the indicated concentrations of SSE for 24 h, and the relative cell viability was assessed using MTT assay. (B) After incubation of cells with the indicated concentrations of SSE, dead cells were counted using trypan blue exclusion assay. The data represent 3 independent experiments carried out in triplicate and expressed as mean ± SD. (C) AGS, B16F10, and murine hepatocytes were incubated with 25 and 50 μg/mL of SSE during the indicated periods and then observed under an inverted microscope.