Figure 4

HDAC inhibitory activity of the plant extracts and sinapinic acid in HeLa cells. Effect of (A) ethanolic crude extract, phenolic-rich extract and (B) sinapinic acid on histone acetylation in HeLa cells. After 6 h-treatment, the level of histone acetylation was determined by AUT gel electrophoresis as described in Materials and Methods. Control (Cont) represents the level of histone acetylation in vehicle control treatment. The sodium butyrate (NaB)-treatment was used as a positive control. The degree of histone acetylation of histone H4 is indicated as follows: 0, nonacetylated; 1, monoacetylated; 2, diacetylated; 3, triacetylated; and 4, tetraacetylated. The data shown are representative of two independent experiments performed in duplicate.