Figure 4

Effect of papain on cell proliferation of HUVEC. HUVEC were seeded into fibronectin coated 96-well microtiterplates and cultured in EGM. After 24 hours medium was changed to EBM containing 10 ng/mL VEGF or 10 ng/mL VEGF and papain at concentrations as indicated. After 72 hours, cell growth was assayed using an MTT-assay kit. Data are shown as optical density at 450 nm with a reference wavelength of 620 nm, mean ± standard error of measurement. Papain exerts a significant inhibition of proliferation at concentrations between 6.25 μg/mL and 100 μg/mL.