Figure 2

RP-HPLC chromatograms. Chromatogram of the ethanolic extract of geopropolis (EEGP) (A) and its hexane fraction (HF) (B) using photodiode array detector (SPD-M10AVp) at 254 nm; reversed-phase C18 column (250 mm × 4.6 mm i.d.; 5 μm particle size); mobile phase: water/acetic acid (19:1, v/v) (solvent A) and methanol (solvent B) at a constant flow rate of 1 mL/min. The gradient started with 30% solvent B, changing to 40% of B in 15 min, 50% of B in 30 min, 60% of B in 45 min, 75% of B in 65 min, 75% of B in 85 min, 90% of B in 95 min, 90% of B in 110 min, and 30% of B in 120 min. The column was maintained at 35°C. Constituents are represented only by the absorption spectra in the UV (λ_max): 1: UV λ 241 nm, RT = 2.72 min; 2: UV λ 287 nm, RT = 81.40 min; 3: UV λ 283 nm, RT = 83.31 min; 4: UV λ 279 nm, RT = 100.53 min; 5: UV λ 281 nm, RT = 101.95 min; 6: UV λ 240 nm, RT = 42.54 min; 7: UV λ 282 nm, RT = 87.14 min; 8: UV λ 284 nm, RT = 91.00 min.