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Figure 1 | BMC Complementary and Alternative Medicine

Figure 1

From: Xiao-Qing-Long-Tang shows preventive effect of asthma in an allergic asthma mouse model through neurotrophin regulation

Figure 1

XQLT inhibited NGF, BDNF, TARC and p75NTR expression in LA4 cells stimulated with Der p. Pre-treatment (XP) and co-treatment (XD) with XQLT in Der p-stimulated cells and merely XQLT treated cells (XC) were used to assess the effects of XQLT on neurotrophin and associated receptor. (A) The supernatant was collected and analyzed by ELISA to obtain NGF, BDNF and TARC levels. Student’s t test was performed versus Group D. Schematic of cell experiment groups is also shown under those ELISA data. (B) Whole cell extracts were used for western blotting with anti-p75NTR antibody or anti-NGF antibody (commercial antibody also recognizes pro-NGF). Protein expression level was assessed as western band density detected by densitometry and was depicted as proportional bar plots (negative group level set as 1). Student’s t test was performed versus Group D. All the experiments in (A) and (B) were repeated for at least three times. The values shown are means ± SD. * means p < 0.05, ** means p < 0.01, *** means p < 0.005 in (A) and (B).

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