Effect of EA preconditioning on tissue and functional outcome. (A) Representative photographs of coronal brain sections following infarction stained with 2,3,5-triphenyltetrazolium chloride. The red area is healthy tissue and the white area is infarct tissue. *, P<0.05 vs. control (Con). (B) Neurological deficit and motor deficit were assessed 24 h after ischemia. Neurological function was assessed by neurological score, vestibule-motor function by a wire grip test, sensori-motor function by a corner test and asymmetry forelimb use for weight shifting by a cylinder test. A regression line showed the relationship between tissue injury (infarct volume) and functional outcome (neurological score, wire grip test, corner test and cylinder test). Results are expressed as mean ± SEM for twelve mice in each group. *, P<0.05 and **, P<0.01 vs. control (Con).