Figure 3From: Estrogenic and mutagenic activities of Crotalaria pallida measured by recombinant yeast assay and Ames test Estrogenic response for stigmasterol, isolated from C. pallida leaves, in the recombinant yeast assay. Different concentrations of stigmasterol, (μg/well) were added to genetically engineered, estrogen- responsive yeast cells and incubated for 6 h. The β-galactosidase activities were calculated as fluorescence units (FU). Values are averages of three independent experiments; bars indicate value ranges. Negative control, DMSO, FU = 54 ± 16; positive control, 17-β-estradiol, FU = 9678 ± 167.Back to article page