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Figure 3 | BMC Complementary and Alternative Medicine

Figure 3

From: Estrogenic and mutagenic activities of Crotalaria pallida measured by recombinant yeast assay and Ames test

Figure 3

Estrogenic response for stigmasterol, isolated from C. pallida leaves, in the recombinant yeast assay. Different concentrations of stigmasterol, (μg/well) were added to genetically engineered, estrogen- responsive yeast cells and incubated for 6 h. The β-galactosidase activities were calculated as fluorescence units (FU). Values are averages of three independent experiments; bars indicate value ranges. Negative control, DMSO, FU = 54 ± 16; positive control, 17-β-estradiol, FU = 9678 ± 167.

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