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Figure 2 | BMC Complementary and Alternative Medicine

Figure 2

From: Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells

Figure 2

Effects of SHT on the cellular tyrosinase activity and melanogenesis-related promoter activation. (A) B16F10 cells were pre-incubated with or without 250 and 500 μg/ml SHT for 12 h, stimulated with 1 μM of α-MSH for additional 36 h, and then cellular tyrosinase activity was determined by measuring the formation of dopachrome from L-DOPA. Visible dopachrome formation was shown in upper panel and relative tyrosinase activity compared with untreated “control” cells was calculated. (B) To determine the effect of SHT on the tyrosinase, CRE and MITF promoter activation, B16F10 cells were transfected with pTyrosinase-luc, pCRE-luc, and pMITF-luc reporter DNA, and incubated for 24 h in the presence or absence of α-MSH and SHT. The data are representative of three independent experiments carried out in triplicate, and expressed as mean ± SD. *p < 0.05 vs control, **p < 0.05 vs α-MSH-treated cells.

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