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Figure 1 | BMC Complementary and Alternative Medicine

Figure 1

From: Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells

Figure 1

Effects of SHT on the cell viability and melanin production in B16F10 cells. B16F10 cells (A) and murine primary hepatocytes (B) were incubated with indicated concentrations of SHT for 48 h, and then the cell viability was determined by MTT assay. (C) B16F10 cells were pre-incubated with or without 250 and 500 μg/ml SHT for 12 h, stimulated with 1 μM of α-MSH for additional 36 h, and then observed for the accumulation of melanin in the microscopic image or pigmentation of cell pellets (2 × 106 cells). Image magnification, ×200. (D) After harvesting cells, cellular melanin content in the same number of cells was determined. Each percentage value was calculated with respect to that of untreated “control” cells as 100%. Data are expressed as the mean ± SD of three independent experiments. *p < 0.05 vs control, **p < 0.05 vs α-MSH-treated cells.

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