Figure 2

Effect of Moringa Oleifera leaf extract on the distribution of Panc-1 cells in the cell cycle. (A) Representative histograms of cell distribution following Moringa Oleifera leaf extract treatments. (B) Data summarized from three independent experiments (%). The cells were treated with the extract for 24 hours, collected, washed and fixed in 70% ice-cold ethanol. Prior to the analysis, the cells were suspended in 0.1% Triton-X with 30 mg/ml DNAse-free RNAse A for 6 hrs. Propidium iodide was added several minutes before cell analysis by flow cytometry. The significance of the effect, relatively to non-treated cells (control), is indicated as *p ≤ 0.05 and **p ≤ 0.001.