Skip to main content

Advertisement

Figure 5 | BMC Complementary and Alternative Medicine

Figure 5

From: Targeting beta-Catenin signaling to induce apoptosis in human breast cancer cells by z-Guggulsterone and Gugulipid extract of Ayurvedic medicine plant Commiphora mukul

Figure 5

Immunoblotting for β-Catenin using lysates from MCF-7 (A) or MDA-MB-231 (C) cells transiently transfected with a control nonspecific siRNA or β-Catenin -targeted siRNA and treated for 24 h with DMSO or 5 μmol/L GL. The blots were stripped and reprobed with anti-actin antibody to ensure equal protein loading. The numbers on top of the immunoreactive bands represent changes in protein levels relative to DMSO-treated nonspecific control siRNA–transfected cells. Cytoplasmic histone-associated apoptotic DNA fragmentation in MCF-7 (B) or MDA-MB-231 (D) cells transiently transfected with a control nonspecific siRNA or β-Catenin -targeted siRNA and treated for 24 h with DMSO or 5 μmol/L GL. The results are expressed as enrichment factor relative to DMSO-treated control cells transiently transfected with the control nonspecific siRNA. Each experiment was done twice, and representative data from a single experiment are shown. Columns, mean (n=3); bars, SE. *, P<0.05, significantly different between the indicated groups by paired t test.

Back to article page