GL (A and C) and z-Gug (B) inhibited β-Catenin level in human breast cancer MCF-7 and MDA-MB-231 cells as well as HMEC. Cells were treated with DMSO (control) or 2.5 and 5 μmol/L GL standardized to z-Gug or 20 and 40 μmol/L z-Gug for 24h. β-Catenin level was determined by using the SurveyorTM IC Human total β-Catenin Immunoassay kit (R&D Systems) following the manufacturer’s instructions. Experiments were repeated twice with triplicate measurements in each experiment. Results are mean±SE (n=3). *Significantly different (P<0.05) between the indicated groups by one-way ANOVA followed by Dennett’s test. The results were consistent and representative data from a single experiment are shown.