In Vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species

Noumedem, Jaurès AK; Mihasan, Marius; Kuiate, Jules R; Stefan, Marius; Cojocaru, Dumitru; Dzoyem, Jean P; Kuete, Victor

doi:10.1186/1472-6882-13-190

BMC Complementary Medicine and Therapies

Table 5 MIC of different antibiotics after the association of the extract of Telfairia occidentalis at MIC/2, MIC/5 against eleven MDR bacteria

From: In Vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species

Antibiotics^a	Bacterial strains^b, MIC (μg/mL ) of antibiotics in the absence and presence of the extract												PBSS^c(%)
Antibiotics^a	Extract concentration	PA124	AG100	AG102	AG100Atet	CM64	EA3	EA27	EA289	BM67	KP55	NEA16	PBSS^c(%)
TET	0	8	16	256	64	8	16	64	16	8	> 256	4
	MIC/2	4(2)^S	8(2)^S	256(1)^I	32(2)^S	≤ 2(> 4)^S	≤ 2(> 8)^S	64(1)^I	8(2)^S	4(2)^S	256(> 1)^s	≤ 2(> 2)^S	81.8
	MIC/5	4(2)^S	8(2)^S	256(1)^I	32(2)^S	≤ 2(> 4)^S	≤ 2(> 8)^S	64(1)^I	8(2)^S	4(2)^S	256(> 1)^s	4(1)^I	72.7
DOX	0	16	8	32	32	32	32	32	32	8	32	64	na
	MIC/2	2(8)^S	≤ 2(> 4)^S	16(2)^S	4(8)^S	4(8)^S	≤ 2(> 16)^S	4(8)^S	16(2)^S	4(2)^S	≤ 2(> 16)^S	32(2)^S	100
	MIC/5	8(2)^S	≤ 2(> 4)^S	32(1)^I	8(4)^S	≤ 2(> 16)^S	≤ 2(> 16)^S	8(4)^S	16(2)^S	≤ 2(> 4)^S	64(< 0.5)	64(1)^I	72.7
CIP	0	32	4	128	64	64	128	≤ 2	8	128	64	128	na
	MIC/2	32(1)^I	≤ 2(> 2)^S	64(2)^S	≤ 2(> 32)^S	16(4)^S	≤ 2(> 64)^S	≤ 2	≤ 2(> 4)^S	128(1)^I	≤ 2(> 32)^S	128(1)^I	70
	MIC/5	32(1)^I	≤ 2(> 2)^S	64(2)^S	≤ 2(> 32)^S	16(4)^S	≤ 2(> 64)^S	≤ 2	≤ 2(> 4)^S	128(1)^I	≤ 2(> 32)^S	128(1)^I	70
NFX	0	128	128	64	128	≤ 2	128	32	64	128	128	256	na
	MIC/2	64(2)^s	4(32)^S	32(2)^S	4(32)^S	≤ 2	4(32)^S	4(8)^S	32(2)^S	128(1)^I	≤ 2(> 64)^S	128(2)^S	90
	MIC/5	32(4)^s	4(32)^S	32(2)^S	4(32)^S	≤ 2	4(32)^S	4(8)^S	16(4)^S	128(1)^I	8(16)^S	256(1)^I	80
STR	0	256	≤ 2	256	64	8	32	16	64	≤ 2	4	64	na
	MIC/2	256(1)^I	≤ 2	256(1)^I	16(4)^S	≤ 2(> 4)^S	≤ 2(> 16)^S	16(1)^I	≤ 2(> 32)^S	≤ 2	≤ 2(> 2)^S	16(4)^S	66.7
	MIC/5	256(1)^I	≤ 2	256(1)^I	8(8)^S	≤ 2(> 4)^S	≤ 2(> 16)^S	16(1)^I	≤ 2(> 32)^S	≤ 2	≤ 2(> 2)^S	16(4)^S	66.7
KAN	0	ND	8	128	32	≤ 2	16	16	32	≤ 2	8	≤ 2	na
	MIC/2	ND	4(2)^S	128(1)^I	8(4)^S	≤ 2	≤ 2(> 8)^S	≤ 2(> 8)^S	≤ 2(> 16)^S	≤ 2	8(1)^I	≤ 2	71.4
	MIC/5	ND	4(2)^S	128 (1)^I	≤ 2(> 16)^S	≤ 2	≤ 2(> 8)^S	≤ 2(> 8)^S	≤ 2(> 16)^S	≤ 2	8(1)^I	≤ 2	71.4
CHL	0	32	64	> 256	64	256	32	> 256	64	16	128	128	na
	MIC/2	16(2)^s	32(2)^S	> 256	64(1)^I	≤ 2(> 128)^S	4(8)^S	256(> 1)^s	16(4)^S	≤ 2(> 8)^S	16(8)^S	16(8)^S	90
	MIC/5	32(1)^I	32(2)^S	> 256	64(1)^I	≤ 2(> 128)^S	8(4)^S	256(> 1)^s	32(2)^S	16(1)^I	64(2)^S	64(2)^S	70
ERY	0	128	32	256	128	> 256	256	64	256	256	256	256	na
	MIC/2	64(2)^s	16(2)^S	> 256	32(4)^S	≤ 2	4(64)^S	32(2)^S	64(4)^S	32(8)^S	8(32)^S	128(2)^S	72.7
	MIC/5	64(2)^s	32(1)^I	> 256	32(4)^S	64(> 4)^S	4(64)^s	64(1)^I	256(1)^I	32(8)^S	16(16)^S	128(2)^S	45.5
AMP	0	128	128	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256	na
	MIC/2	256(0.5) ^I	128(1)^I	> 256	> 256	> 256	256(> 1)^s	> 256	> 256	> 256	> 256	> 256	na
	MIC/5	256(0.5) ^I	128(1)^I	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256	na
FEP	0	ND	> 256	> 256	> 256	> 256	> 256	> 256	> 256	256	> 256	> 256	na
	MIC/2	ND	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256(< 1)	8(> 32)^S	> 256	1.1
	MIC/5	ND	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256(< 1)	> 256	> 256	na
CLX	0	> 256	256	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256	> 256	na
CLX	MIC/2	> 256	64(> 4)^S	> 256	> 256	256(≥2) ^S	64(> 4)^S	> 256	> 256	> 256	> 256	> 256	27.3
	MIC/5	> 256	64(> 4)^S	> 256	> 256	256(≥2) ^S	128(> 2)^S	> 256	> 256	> 256	> 256	> 256	27.3

^aAntibotics [TET : tetracycline, DOX: doxycyclin, CIP: ciprofloxacin, NOR : norfloxacin, STR : streptomycin, KAN : kanamycin, CHL: chloramphenicol, ERY : erythromycin, AMP : ampicillin, FEP: cefepime, CLX: cloxacillin]. ^bBacterial strains: Escherichia coli [AG100, AG100A, AG100Atet], Pseudomonas aeruginosa [PA124], Enterobacter aerogenes [CM64, EA3, EA27, EA289], Enterobacter cloacae [BM67], Klebsiella pneumoniae [KP55], Providencia stuartii [NAE16]. ^cPBSS: percentage of bacteria strain on which synergism has been observed; (): fold increase in MIC values of the antibiotics after association with plants extract; S: Synergy, I: Indifference; na: not applicable tract; S: Synergy, I: Indifference; na: not applicable.

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