Figure 7

Effect of STB-HO on pVEGFR2, PI3K and Akt in HUVECs. HUVECs were starved for 24 h in M199 containing 1% heat-inactivated FBS and then treated with various concentrations of STB-HO (0, 250 or 500 μg/ml) in M199 containing 1% heat-inactivated FBS, 10 ng/ml VEGF and 5 units/ml heparin for 10 min, 30 min, 1 h, 2 h, and 24 h. The cells were harvested and western blotting was performed to determine the expression of pVEGFR2, VEGFR2, pAKT, AKT and β-actin.