Figure 3

Effect of STB-HO on G1 arrest and cell cycle related genes in HCT116 cells. (A) Cells were treated with STB-HO (0, 250 or 500 μg/ml) for 24, 48 and 72 h. Cell cycle distribution was analyzed by flow cytometry. Bar graphs represent the percentage of the sub-G1 apoptotic DNA fraction. Data are presented as means ± S.D. *p < 0.05 and ***p < 0.001 compared between each time’s control and STB-HO (250 or 500 μg/ml) treated groups. (B) HCT116 cells were treated with or without STB-HO (0, 250 or 500 μg/ml) for 24 h. Cell lysates were prepared and subjected to Western blotting to determine the expression of p21, p27, p53, pp53, Cyclin D1, PCNA and β-actin. Band density of β-actin, p21, p27, pp53, PCNA and Cyclin D1 were quantified using Gelpro analyzer (Media Cybernetics, Bethesda, MD, USA).