Effects of GTPs on the expression of claudin-5, occludin, ZO-1, and PKCα mRNA at the early stage of brain ischemia were determined by RT-PCR. (A) M, DNA ladder; V0–V120, 0, 30, 60, and 120 min after ischemia in the vehicle group; G0–G120, 0, 30, 60, and 120 min after ischemia in the GTPs-treated group. The mRNA levels of the gene expression were normalized to those of β-Actin as an endogenous reference. (B-E) IDV of claudin-5, occludin, ZO-1, and PKCα is expressed as a fold reduction or a fold increase relative to the vehicle group of ischemia 0 min. Data represent the means ± SD (n = 6, each). ** P < 0.01 vs. vehicle group of ischemia at 0 min. #
P < 0.05, ##
P < 0.01 vs. vehicle group at the same time points.