Effects of bufalin on GSK3β, β-catenin levels, and nuclear translocation of β-catenin. Protein levels of pGSK3β (A, C) and β-catenin (B, D) were analyzed by western blotting in bufalin-treated (100 nmol/L) hepatoma cells. LY294002 (10 μmol/L) was included as a positive control. GAPDH was used as a loading control. Nuclear translocation of β-catenin in bufalin- and LY29400-treated hepatoma cells (E, F) was monitored by immunofluorescence assay. Columns represent the mean of three individual experiments performed in triplicate; error bars represent SD; LY represent LY294002. **P < 0.01, vs. controls.