Effects of bufalin on AKT and pAKT (Ser 473) expression in hepatoma cells. (A) HCCLM3 and HepG2 cells were treated with bufalin (100 nmol/L) and LY294002 (10 mmol/L) for the indicated times. GAPDH was used as a loading control. (B) Quantitative analysis of expression of AKT and pAKT (Ser 473). Columns represent the mean of three individual experiments performed in triplicate; error bars represent SD; LY represent LY294002. **P < 0.01, vs. controls.